LINC00636 promotes lymph node metastasis and cervical cancer through targeting NM23.

ABSTRACT: BACKGROUND:Metastasis is a major obstacle in treatment of cervical cancer, and long non-coding RNA (lncRNA) mediated regulatory effect on associated genes expression is found to be involved in metastasis. However, its mechanisms have not been fully elucidated. MATERIALS AND METHODS:Specimens from patients with cervical cancer metastasis and non-metastasis were used to screen out candidate non-coding RNAs (ncRNAs) and possible downstream targets. And then, effects were determined in vitro and in vivo through knockdown and overexpression techniques. RESULTS:LINC00636 was significantly higher in serum and solid tumor cells of metastatic cervical cancer patients than non-metastatic patients. And knockdown of LINC00636 significantly suppressed invasion, proliferation of cervical cancer cells. NM23 expression was negatively regulated by LINC00636 and it mediated anti-tumor effects was partially blocked by overexpression of LINC00636. CONCLUSION:LINC00636 might promote metastasis of cervical cancer cells through inhibiting NM23 expression.

SUBMITTER: Zhong Y 

PROVIDER: S-EPMC7601350 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature