Detargeting Lentiviral-Mediated CFTR Expression in Airway Basal Cells Using miR-106b.

ABSTRACT: Lentiviral-mediated integration of a CFTR transgene cassette into airway basal cells is a strategy being considered for cystic fibrosis (CF) cell-based therapies. However, CFTR expression is highly regulated in differentiated airway cell types and a subset of intermediate basal cells destined to differentiate. Since basal stem cells typically do not express CFTR, suppressing the CFTR expression from the lentiviral vector in airway basal cells may be beneficial for maintaining their proliferative capacity and multipotency. We identified miR-106b as highly expressed in proliferating airway basal cells and extinguished in differentiated columnar cells. Herein, we developed lentiviral vectors with the miR-106b-target sequence (miRT) to both study miR-106b regulation during

SUBMITTER: Choi SH 

PROVIDER: S-EPMC7601932 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature