A novel cell permeability assay for macromolecules.

ABSTRACT:

Background

Many cell permeabilisation methods to mediate internalisation of various molecules to mammalian or bacterial cells have been developed. However, no size-specific permeability assay suitable for both cell types exists.

Results

We report the use of intrinsically biotinylated cell components as the target for reporter molecules for assessing permeabilisation. Due to its well-described biotin binding activity, we developed an assay using Streptavidin (SAv) as a molecular weight marker for assessing eukaryotic and prokaryotic cell internalisation, using flow cytometry as a readout. This concept was tested here as part of the development of host DNA depletion strategies for microbiome analysis of formalin-fixed (FF) samples. Host depletion (HD) strategies require differ

SUBMITTER: Flores Bueso Y 

PROVIDER: S-EPMC7602297 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature