ER-to-Golgi Transport in HeLa Cells Displays High Resilience to Ca²⁺ and Energy Stresses.

ABSTRACT: One third of all human proteins are either transmembrane or soluble secretory proteins that first target the endoplasmic reticulum (ER). These proteins subsequently leave the ER and enter the Golgi apparatus via ER-Golgi intermediate vesicular structures. Live-cell imaging of cargos fused to fluorescent proteins (FPs) enables the high-resolution visualization and characterization of secretory transport processes. Here, we performed fluorescence time-lapse imaging to assess the Ca²⁺ and energy dependency of ER-to-Golgi transport in living HeLa cells, a cancer cell model which has been well investigated. Our data revealed that ER-to-Golgi transport remained highly efficient in the absence of ATP-generating substrates, despite clear reductions in cytosolic and mitochondrial ATP lev

SUBMITTER: Rauter T 

PROVIDER: S-EPMC7603030 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature