Novel Inter-omic Analysis Reveals Relationships Between Diverse Gut Microbiota and Host Immune Dysregulation in HLA-B27-Induced Experimental Spondyloarthritis.

ABSTRACT:

Objective

To define inflammation-related host-microbe interactions in experimental spondyloarthritis (SpA) using novel inter-omic approaches.

Methods

The relative frequency of gut microbes was determined by 16S ribosomal RNA (rRNA) gene sequencing, and gene expression using RNA-Seq of host tissue. HLA-B27/human β₂ -microglobulin-transgenic (HLA-B27-transgenic) and wild-type rats from dark agouti, Lewis, and Fischer backgrounds were used. Inter-omic analyses using Cytoscape were employed to identify relevant relationships. PICRUSt was used to predict microbial functions based on known metagenomic profiles.

Results

Inter-omic analysis revealed several gut microbes that were strongly associated with dysregulated cytokines driving inflammatory response pathways

SUBMITTER: Gill T 

PROVIDER: S-EPMC7603391 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature