Handling deviating control values in concentration-response curves.

ABSTRACT: In cell biology, pharmacology and toxicology dose-response and concentration-response curves are frequently fitted to data with statistical methods. Such fits are used to derive quantitative measures (e.g. EC[Formula: see text] values) describing the relationship between the concentration of a compound or the strength of an intervention applied to cells and its effect on viability or function of these cells. Often, a reference, called negative control (or solvent control), is used to normalize the data. The negative control data sometimes deviate from the values measured for low (ineffective) test compound concentrations. In such cases, normalization of the data with respect to control values leads to biased estimates of the parameters of the concentration-response curve. Low quality estim

SUBMITTER: Kappenberg F 

PROVIDER: S-EPMC7603474 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature