Bacteria and sputum inflammatory cell counts; a COPD cohort analysis.

ABSTRACT:

Background

There is evidence that bacterial colonisation in chronic obstructive pulmonary disease (COPD) is associated with increased neutrophilic airway inflammation. This study tested the hypothesis that different bacterial phyla and species cause different inflammatory profiles in COPD patients.

Methods

Sputum was analysed by quantitative polymerase chain reaction (qPCR) to quantify bacterial load and 16S rRNA gene sequencing to identify taxonomic composition. Sputum differential cell counts (DCC) and blood DCC were obtained at baseline and 6 months. Patients were categorised into five groups based on bacterial load defined by genome copies/ml of ≥ 1 × 10⁴, no colonisation and colonisation by Haemophilus influenzae (H. influenzae), Moraxella catarrhalis (M. cat

SUBMITTER: Beech AS 

PROVIDER: S-EPMC7603729 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature