The eukaryotic translation initiation factor eIF4E elevates steady-state m⁷G capping of coding and noncoding transcripts.

ABSTRACT: Methyl-7-guanosine (m⁷G) "capping" of coding and some noncoding RNAs is critical for their maturation and subsequent activity. Here, we discovered that eukaryotic translation initiation factor 4E (eIF4E), itself a cap-binding protein, drives the expression of the capping machinery and increased capping efficiency of ∼100 coding and noncoding RNAs. To quantify this, we developed enzymatic (cap quantification; CapQ) and quantitative cap immunoprecipitation (CapIP) methods. The CapQ method has the further advantage that it captures information about capping status independent of the type of 5' cap, i.e., it is not restricted to informing on m⁷G caps. These methodological advances led to unanticipated revelations: 1) Many RNA populations are inefficiently capped at steady

SUBMITTER: Culjkovic-Kraljacic B 

PROVIDER: S-EPMC7604501 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature