Indigenous Medicinal Plants as Biofilm Inhibitors for the Mitigation of Antimicrobial Resistance.
Ontology highlight
ABSTRACT:
SUBMITTER: Ampofo EK
PROVIDER: S-EPMC7604581 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Publications
Indigenous Medicinal Plants as Biofilm Inhibitors for the Mitigation of Antimicrobial Resistance.
Ampofo Elikplim Kwesi EK Amponsah Isaac Kingsley IK Asante-Kwatia Evelyn E Armah Francis Ackah FA Atchoglo Philip Kobla PK Mensah Abraham Yeboah AY
Advances in pharmacological and pharmaceutical sciences 20201024
The majority of indigenes in the rural areas of Ghana use herbal medicines for their primary health care. In this study, an ethnobotanical survey was undertaken to document medicinal plants used by traditional healers in the Ejisu-Juaben district in the Ashanti region of Ghana to treat infections and to further investigate the antibiofilm formation properties of selected plants in resisting pathogenic bacteria. Seventy medicinal plants used by traditional practitioners for the treatment of skin ...[more]
Similar Datasets
Project description:For a long time, the people of Saudi Arabia have been using medicinal plants (MPs) as conventional medicine to heal diverse human and livestock diseases. The present work is the first study on ethnobotanical uses of 124 MPs species used by the local tribal communities of Jazan province in the Southwest of Saudi Arabia. Ethnobotanical data were collected by interviewing 174 local informants using semistructured interviews. Informants of different ages, from several settlements belonging to several tribal communities, were interviewed. It is worth noticing that the age of informants and their knowledge of MPs were positively correlated, whereas the educational level and MP knowledge of participants were negatively correlated. To find out if there was agreement in the use of certain plants in the treatment of given ailments, we used Informant Consensus Factor (ICF). To determine the most frequently used plant species for treating a particular ailment category by local people we used the fidelity level (FL%). The Relative Frequency of Citation (RFC) was used to indicate the local importance of a species and the relative importance (RI) level was used to check the therapeutic potentials of the cited plants. A total of 124 MPs belonging to 103 genera and 48 families were collected and identified. The majority of these plants were shrubs (45%), perennial herbs (21%), annual herbs (19%), or trees (18%). The Asteraceae (10.48%), Fabaceae (7.25%), and Apocynaceae (7.25%) families were the most represented. Leaves, fruits, and whole plant (24%, 18%, and 16%, respectively) were the most used plant parts in formulating traditional medicines. Ziziphus spina-christi and Calotropis procera with the highest RI level (2.0) were found to have the highest range of therapeutic uses. They were followed by Datura stramonium (1.86), Withania somnifera, and Aloe vera (1.81). The ICF ranged from 0.02 to 0.42 covering 12 disease categories with a prevalence of disease categories related to skin and hair problems (ICF=0.42) having 75 species cited, while 73 species were cited for gastrointestinal tract (GIT) disorders (ICF = 0.40). Senna alexandrina (67%), Tribulus terrestris (64%), Pulicaria undulata (60%), Leptadenia pyrotechnica (55%), and Rumex nervosus (55%) had the highest FL which indicates their good healing potential against specific diseases. The high-FL species are the most promising candidate plants for in-depth pharmacological screening and merit further consideration. Accordingly, Jazan flora has good ethnobotanical potential. Unfortunately, many MP species are endangered by drought, overgrazing, and overexploitation. Some protection measures should be undertaken to prevent these species from becoming extinct. Natural reserves and wild nurseries are typical settings to retain medically important plants in their natural habitats, while botanic gardens and seed banks are important paradigms for ex situ conservation.
| S-EPMC6582903 | biostudies-literature
Project description:Present paper offers considerable information on traditional uses of medicinal plants by the inhabitants of Hafizabad district, Punjab-Pakistan. This is the first quantitative ethnobotanical study from the area comprising popularity level of medicinal plant species intendedby using relative popularity level (RPL) and rank order priority (ROP) indices.Ethnobotanical data were collected by interviewing 166 local informants and 35 traditional health practioners (THPs) from different localities of Hafizabad district. Demographic features of informants; life form, part used, methods of preparation, modes of application and ethnomedicinal uses were documented. Ethnobotanical data were analyzed using quantitative tools, i.e. Relative frequency citation (RFC), use value (UV), informant consensus factor (ICF) fidelity level (FL), RPL and ROP indices. A total of 85 species belonging to 71 genera and 34 families were documented along with ethnomedicinal uses. Solanum surattense, Withania somnifera, Cyperus rotundus, Solanum nigrum and Melia azedarach were the most utilized medicinal plant species with highest used value. The reported ailments were classified into 11 disease categories based on ICF values and highest number of plant species was reported to treat dermatological and gastrointestinal disorders. Withania somnifera and Ranunculus sceleratus with maximum FL (100%), were used against gastrointestinal and urinary disorders, respectively. The RPL and ROP values were calculated to recognize the folk medicinal plant wealth; six out of 32 plant species (19%) were found popular, based on citation by more than half of the maximum number of informant viz. 26. Consequently, the ROP value for these species was more than 75. The comparative assessment with reported literature revealed 15% resemblance and 6% variation to previous data;however79% uses of the reported species were recorded for the first time. The diversity of medicinal plant species and associated traditional knowledge is significant in primary health care system. Medicinal plant species with high RPL values should be screened for comprehensive phytochemical and pharmacological studies. This could be useful in novel drug discovery and to validate the ethomendicinal knowledge.
| S-EPMC5456064 | biostudies-literature
Project description:Natural products have been the center of attraction ever since they were discovered. Among them, plant-based natural products were popular as analgesics, anti-inflammatory, antidiabetic, and cosmetics and possess widespread biotechnological applications. The use of plant products as cosmetics and therapeutics is deep-rooted in Nepalese society. Although there are few ethnobotanical studies conducted, extensive research of these valuable medicinal plants has not been a priority due to the limitation of technology and infrastructure. Here, we selected 4 traditionally used medicinal plants to examine their bioactive properties and their enzyme inhibition potential. α-Glucosidase and α-amylase inhibitory activities were investigated using an in vitro model followed up by antioxidant and antimicrobial activities. The present study shows that ethyl acetate fraction of Melastoma melabathrium (IC50 9.1 ± 0.3 µg/mL) and water fraction Acacia catechu (IC50 9.0 ± 0.6 µg/mL) exhibit strong α-glucosidase inhibition. Likewise, the highest α-amylase inhibition was shown by crude extracts of Ficus religiosa (IC50 29.2 ± 1.2 µg/mL) and ethyl acetate fractions of Shorea robusta (IC50 69.3 ± 1.1 µg/mL), and the highest radical scavenging activity was shown by F. religiosa with an IC50 67.4 ± 0.6 µg/mL. Furthermore, to identify the metabolites within the fractions, we employed high-resolution mass spectrometry (LC-HRMS) and annotated 17 known metabolites which justify our assumption on activity. Of 4 medicinal plants examined, ethyl acetate fraction of S. robusta, ethyl acetate fraction of M. melabathrium, and water or ethyl acetate fraction of A. catechu extracts illustrated the best activities. With our study, we set up a foundation that provides authentic evidence to the community for use of these traditional plants. The annotated metabolites in this study support earlier experimental evidence towards the inhibition of enzymes. Further study is necessary to explore the clinical efficacy of these secondary molecules, which might be alternatives for the treatment of diabetes and pathogens.
| S-EPMC8121587 | biostudies-literature
Project description:BackgroundBy revealing historical and present plant use, ethnobotany contributes to drug discovery and socioeconomic development. Nepal is a natural storehouse of medicinal plants. Although several ethnobotanical studies were conducted in the country, many areas remain unexplored. Furthermore, few studies have compared indigenous plant use with reported phytochemical and pharmacological properties.MethodsEthnopharmacological data was collected in the Rasuwa district of Central Nepal by conducting interviews and focus group discussions with local people. The informant consensus factor (FIC) was calculated in order to estimate use variability of medicinal plants. Bio-efficacy was assessed by comparing indigenous plant use with phytochemical and pharmacological properties determined from a review of the available literature. Criteria were used to identify high priority medicinal plant species.ResultsA total of 60 medicinal formulations from 56 plant species were documented. Medicinal plants were used to treat various diseases and disorders, with the highest number of species being used for gastro-intestinal problems, followed by fever and headache. Herbs were the primary source of medicinal plants (57% of the species), followed by trees (23%). The average FIC value for all ailment categories was 0.82, indicating a high level of informant agreement compared to similar studies conducted elsewhere. High FIC values were obtained for ophthalmological problems, tooth ache, kidney problems, and menstrual disorders, indicating that the species traditionally used to treat these ailments are worth searching for bioactive compounds: Astilbe rivularis, Berberis asiatica, Hippophae salicifolia, Juniperus recurva, and Swertia multicaulis. A 90% correspondence was found between local plant use and reported plant chemical composition and pharmacological properties for the 30 species for which information was available. Sixteen medicinal plants were ranked as priority species, 13 of which having also been prioritized in a country-wide governmental classification.ConclusionsThe Tamang people possess rich ethnopharmacological knowledge. This study allowed to identify many high value and high priority medicinal plant species, indicating high potential for economic development through sustainable collection and trade.
| S-EPMC2823594 | biostudies-literature
Project description:BackgroundThe study aimed at documenting the indigenous and local knowledge and use of traditional medicinal plants for treating human and livestock ailments in Dawuro Zone of Ethiopia.MethodsA survey was conducted among traditional healers and native administrators through discussion, interviews, and field observations. The snowball sampling technique was used to select 384 traditional healers in purposefully selected 50 villages spanning seven districts for face-to-face individual interviews. The chi-square test was applied to establish associations between traditional healers' demographics, the distance between the village site and the nearest natural forest and a health center, and SPSS V.20 software was used for the analysis.ResultsThe traditional healers of the study area reported the use of 274 traditional medicinal plant species belonging to 217 genera and 82 families. Asteraceae (11.68%), Fabaceae (9.49%), and Lamiaceae (9.12%) were the foremost frequently used families. Herb species (54.8%) and leaves (65%) were predominantly sourced from the wild environment. The quantity of medicinal plants used (x2 = 278.368, df = 20, P = 0.000) and years of (experience in) traditional healing using herbs (x2 = 76.358, df = 10, P = 0.000) varied with distance from the natural forests. The service charge for healing had strong positive association (x2 = 24.349, df = 5, P = 0.000) with healer's age (x2 = 309.119, df = 184, P = 0.000) and educational level (x2 = 851.230, df = 598, P = 0.000) with distance of traditional healer's residence from the medical institution. The agricultural activities, urbanization, low or no charge for the healing service, the secrecy and oral transfer of the knowledge, and the demand for medicinal and other multiple purposes species were some of the factors threatening the resource and the associated knowledge as well as the service in the study area.ConclusionThere are diversified traditional medicinal plants applied for healthcare of the community and domestic animals of the study area. The source of remedies mostly depends on herbs of natural forests, and the leaf was the most frequently used plant part. Developing conservation intervention and sustainable systems of utilization is needed for multipurpose medicinal plants. Finally, integrating with modern system and formalizing, legalizing, and capacitating the traditional medicine practitioners are needed for access of primary healthcare systems to rural communities.
| S-EPMC9210772 | biostudies-literature
Project description:BackgroundMalaria remains the leading cause of death in sub-Saharan Africa. Although recent developments such as malaria vaccine trials inspire optimism, the search for novel antimalarial drugs is urgently needed to control the mounting resistance of Plasmodium species to the available therapies. The present study was conducted to document ethnobotanical knowledge on the plants used to treat symptoms of malaria in Tororo district, a malaria-endemic region of Eastern Uganda.MethodsAn ethnobotanical study was carried out between February 2020 and September 2020 in 12 randomly selected villages of Tororo district. In total, 151 respondents (21 herbalists and 130 non-herbalists) were selected using multistage random sampling method. Their awareness of malaria, treatment-seeking behaviour and herbal treatment practices were obtained using semi-structured questionnaires and focus group discussions. Data were analysed using descriptive statistics, paired comparison, preference ranking and informant consensus factor.ResultsA total of 45 plant species belonging to 26 families and 44 genera were used in the preparation of herbal medicines for management of malaria and its symptoms. The most frequently mentioned plant species were Vernonia amygdalina, Chamaecrista nigricans, Aloe nobilis, Warburgia ugandensis, Abrus precatorius, Kedrostis foetidissima, Senna occidentalis, Azadirachta indica and Mangifera indica. Leaves (67.3%) were the most used plant part while maceration (56%) was the major method of herbal remedy preparation. Oral route was the predominant mode of administration with inconsistencies in the posology prescribed.ConclusionThis study showed that the identified medicinal plants in Tororo district, Uganda, are potential sources of new antimalarial drugs. This provides a basis for investigating the antimalarial efficacy, phytochemistry and toxicity of the unstudied species with high percentage use values to validate their use in the management of malaria.
| S-EPMC10258082 | biostudies-literature
Project description:This study documents information on significant ethnomedicinal plants, which was collected from the traditional healers of three indigenous communities of Bangladesh. The documented data were quantitatively analyzed for the first time in this area. The information was obtained through open-ended, semi-structured questionnaires. The benefits, importance and coverage of ethnomedicine were expressed through several quantitative indices including Informant Consensus Factor (ICF), Use Value (UV), Frequency of Citation (FC), Relative Frequency of Citation (RFC) and Relative Importance Index (RI). The agreement of homogeneity between the present and previous studies and among the indigenous communities was evaluated using the Jaccard Index (JI). A total of 159 ethnomedicinal plant species, which were distributed in 132 genera under 62 families, were documented from 174 informants. Of these, 128 plants were native and 31 were exotic. Of a majority of documented species, herbs and leaves were the most utilized plant parts for the preparation of ethnomedicines (45.28%) whereas pastes (63.03%) were the most popular formulations. Among the documented species, the dominant families were the Asteraceae (14 species) and the Lamiaceae (12 species). The highest ICF value was 0.77 for digestive system disorders. Based on UVs, the five most commonly used ethnomedicinal plant species in the study area were Duabanga grandiflora (0.43), Zingiber officinale (0.41), Congea tomentosa (0.40), Matricaria chamomilla (0.33) and Engelhardtia spicata (0.28). The highest RFC was recorded for Rauvolfia serpentina (0.25). The highest RI value was calculated for both Scoparia dulcis and Leucas aspera (0.83). Importantly, 16 species were reported with new therapeutic uses and to our knowledge, 7 species described herein have never been ethnobotanically and pharmacologically studied, viz: Agastache urticifolia, Asarum cordifolium, C. tomentosa, E. spicata, Hypserpa nitida, Merremia vitifolia and Smilax odoratissima. The present study showed that traditional treatment using medicinal plants is still widespread in the study area. Documentation of new ethnomedicinal species with their therapeutic uses shall promote further phytochemical and pharmacological investigations and possibly, lead to the development of new drugs.
| S-EPMC5808248 | biostudies-literature
Project description:BackgroundPlants have traditionally been used as a source of medicine in India by indigenous people of different ethnic groups inhabiting various terrains for the control of various ailments afflicting human and their domestic animals. The indigenous community of snake charmers belongs to the 'Nath' community in India have played important role of healers in treating snake bite victims. Snake charmers also sell herbal remedies for common ailments. In the present paper an attempt has been made to document on ethno botanical survey and traditional medicines used by snake charmers of village Khetawas located in district Jhajjar of Haryana, India as the little work has been made in the past to document the knowledge from this community.MethodsEthno botanical data and traditional uses of plants information was obtained by semi structured oral interviews from experienced rural folk, traditional herbal medicine practitioners of the 'Nath' community. A total of 42 selected inhabitants were interviewed, 41 were male and only one woman. The age of the healers was between 25 years and 75 years. The plant specimens were identified according to different references concerning the medicinal plants of Haryana and adjoining areas and further confirmation from Forest Research Institute, Dehradun.ResultsThe present study revealed that the people of the snake charmer community used 57 medicinal plants species that belonged to 51 genera and 35 families for the treatment of various diseases. The study has brought to light that the main diseases treated by this community was snakebite in which 19 different types of medicinal plants belongs to 13 families were used. Significantly higher number of medicinal plants was claimed by men as compared to women. The highest numbers of medicinal plants for traditional uses utilized by this community were belonging to family Fabaceae.ConclusionThis community carries a vast knowledge of medicinal plants but as snake charming is banned in India as part of efforts to protect India's steadily depleting wildlife, this knowledge is also rapidly disappearing in this community. Such type of ethno botanical studies will help in systematic documentation of ethno botanical knowledge and availing to the scientific world plant therapies used as antivenin by the Saperas community.
| S-EPMC2826346 | biostudies-literature
Project description:Kakamega forest is Kenya's only rainforest and is distinguishably rich in biodiversity but threatened by agricultural encroachment and other forms of human activity. It is also one of Kenya's Important Bird Areas and a significant source of natural products to neighboring rural communities, such as medicinal plants, food, wood and other fibers. By using structured questionnaires for direct interviews, local indigenous knowledge was tapped through involvement of a focal group of elderly key informants in three blocks of the forest. Forty key species of medicinal plants used by local people were identified and recorded. Fifty-five percent of these were shrubs, thirty-two percent trees, seven-and-a-half percent lower plants such as herbs or forbs while five percent were climbers. About seventy percent of the medicinal plants occurred inside the forest itself and thirty percent around the edge and the immediate surroundings outside the forest. Thirty-eight (95%) of the plants were indigenous to Kenya and two (5%) exotic. Such extensive indigenous knowledge of the medicinal uses of the plants, including their distribution trends in the forest, may be tapped for decision support in rural health service planning, policy formulation for conserving the forest, tracking and mitigation of climate change impacts.
| S-EPMC3954169 | biostudies-literature
Project description:BackgroundThe Sto. Niño site in Benguet province, Philippines was once a mining area that has now been transformed into an agricultural land. In this area, there has been significant integration of the three indigenous people (IPs) Ibaloi, Kankanaeys and Kalanguyas with the Ilocano community. These IPs safeguard biodiversity and traditional knowledge, including medicinal plant use. However, the documentation of these plant species and their medicinal applications has not been systematic, with the resultant loss of knowledge across generations. This study aims to document the medicinal and ritual plants used by the indigenous communities at the site, in order to preserve and disseminate traditional medicinal knowledge that would otherwise be lost.MethodsEthnobotanical data were collected in Sto. Niño, Brgy. Ambassador, Municipality of Tublay, Benguet, Philippines, and collected through semi-structured interviews, together with focus group discussions (FGD). A total of 100 residents (39 male and 61 female) were interviewed. Among them, 12 were key interviewees, including community elders and farmers, while the rest were selected through the convenience and snowball technique. Demographic information collected from the interviewees included age, gender, and occupation. Ethnobotanical information collected focused on medicinal plants, including the specific parts of plants used, methods of preparation, modes of treatment, and the types of ailments treated. Ethnobotanical quantitative indices of the relative frequency of citations (RFC) and informant consensus factor (ICF) were calculated to evaluate the plant species that were utilized by the community.ResultsA total of 28 medicinal plants from 20 different families and 6 ritual plants from 5 different families were documented. Asteraceae, Poaceae, and Lamiaceae (10.71%) family are the most mentioned medicinal plant species, followed by Myrtaceae and Euphorbiaceae (7.14%). The most widely used growth form were herbs (46.4%), while leaves (61.5%) were the most utilized plant part, and the preparation of a decoction (62.2%) was the most preferred method of processing and application. The medicinal plants were most commonly utilized for wound-healing, cough and colds, stomachache and kidney trouble, whereas ritual plants were largely used for healing, protection, and funeral ceremonies.ConclusionThis study marks the first report on the medicinal and ritual plants used by a group of indigenous communities in Sto. Niño, Brgy. Ambassador, Tublay, Benguet Province. The data collected show that plant species belonging to the Asteraceae, Poaceae, and Lamiaceae family were the most mentioned and should be further evaluated by pharmacological analysis to assess their wider use for medicinal treatment.
| S-EPMC11385124 | biostudies-literature