Biases in genome reconstruction from metagenomic data.

ABSTRACT:

Background

Advances in sequencing, assembly, and assortment of contigs into species-specific bins has enabled the reconstruction of genomes from metagenomic data (MAGs). Though a powerful technique, it is difficult to determine whether assembly and binning techniques are accurate when applied to environmental metagenomes due to a lack of complete reference genome sequences against which to check the resulting MAGs.

Methods

We compared MAGs derived from an enrichment culture containing ~20 organisms to complete genome sequences of 10 organisms isolated from the enrichment culture. Factors commonly considered in binning software-nucleotide composition and sequence repetitiveness-were calculated for both the correctly binned and not-binned regions. This direct comparison reveal

SUBMITTER: Nelson WC 

PROVIDER: S-EPMC7605220 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature