Ontology highlight
ABSTRACT:
SUBMITTER: Kawata K
PROVIDER: S-EPMC7605267 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Kawata Kentaro K Wakida Hiroyasu H Yamada Toshimichi T Taniue Kenzui K Han Han H Seki Masahide M Suzuki Yutaka Y Akimitsu Nobuyoshi N
Genome research 20200825 10
Gene expression is determined by a balance between RNA synthesis and RNA degradation. To elucidate the underlying regulatory mechanisms and principles of this, simultaneous measurements of RNA synthesis and degradation are required. Here, we report the development of "Dyrec-seq," which uses 4-thiouridine and 5-bromouridine to simultaneously quantify RNA synthesis and degradation rates. Dyrec-seq enabled the quantification of RNA synthesis and degradation rates of 4702 genes in HeLa cells. Functi ...[more]