Identification of determinants of differential chromatin accessibility through a massively parallel genome-integrated reporter assay.

ABSTRACT: A key mechanism in cellular regulation is the ability of the transcriptional machinery to physically access DNA. Transcription factors interact with DNA to alter the accessibility of chromatin, which enables changes to gene expression during development or disease or as a response to environmental stimuli. However, the regulation of DNA accessibility via the recruitment of transcription factors is difficult to study in the context of the native genome because every genomic site is distinct in multiple ways. Here we introduce the multiplexed integrated accessibility assay (MIAA), an assay that measures chromatin accessibility of synthetic oligonucleotide sequence libraries integrated into a controlled genomic context with low native accessibility. We apply MIAA to measure the effects of seq

SUBMITTER: Hammelman J 

PROVIDER: S-EPMC7605270 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature