State of the Art in the Culture of the Human Microbiota: New Interests and Strategies.

ABSTRACT: The last 5 years have seen a turning point in the study of the gut microbiota with a rebirth of culture-dependent approaches to study the gut microbiota. High-throughput methods have been developed to study bacterial diversity with culture conditions aimed at mimicking the gut environment by using rich media such as YCFA (yeast extract, casein hydrolysate, fatty acids) and Gifu anaerobic medium in an anaerobic workstation, as well as media enriched with rumen and blood and coculture, to mimic the symbiosis of the gut microbiota. Other culture conditions target phenotypic and metabolic features of bacterial species to facilitate their isolation. Preexisting technologies such as next-generation sequencing and flow cytometry have also been utilized to develop innovative methods to isolate pre

SUBMITTER: Tidjani Alou M 

PROVIDER: S-EPMC7605308 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature