Single cell transcriptomics of mouse kidney transplants reveals a myeloid cell pathway for transplant rejection.

ABSTRACT: Myeloid cells are increasingly recognized as major players in transplant rejection. Here, we used a murine kidney transplantation model and single cell transcriptomics to dissect the contribution of myeloid cell subsets and their potential signaling pathways to kidney transplant rejection. Using a variety of bioinformatic techniques, including machine learning, we demonstrate that kidney allograft-infiltrating myeloid cells followed a trajectory of differentiation from monocytes to proinflammatory macrophages, and they exhibited distinct interactions with kidney allograft parenchymal cells. While this process correlated with a unique pattern of myeloid cell transcripts, a top gene identified was Axl, a member of the receptor tyrosine kinase family Tyro3/Axl/Mertk (TAM). Using kidney transp

SUBMITTER: Dangi A 

PROVIDER: S-EPMC7605544 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature