Rapid SARS-CoV-2 testing in primary material based on a novel multiplex RT-LAMP assay.

ABSTRACT:

Background

Rapid and extensive testing of large parts of the population and specific subgroups is crucial for proper management of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections and decision-making in times of a pandemic outbreak. However, point-of-care (POC) testing in places such as emergency units, outpatient clinics, airport security points or the entrance of any public building is a major challenge. The need for thermal cycling and nucleic acid isolation hampers the use of standard PCR-based methods for this purpose.

Methods

To avoid these obstacles, we tested PCR-independent methods for the detection of SARS-CoV-2 RNA from primary material (nasopharyngeal swabs) including reverse transcription loop-mediated isothermal amplification (RT-LAMP) an

SUBMITTER: Schermer B 

PROVIDER: S-EPMC7605681 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature