Specific induction and long-term maintenance of high purity ventricular cardiomyocytes from human induced pluripotent stem cells.

ABSTRACT: Currently, cardiomyocyte (CM) differentiation methods require a purification step after CM induction to ensure the high purity of the cell population. Here we show an improved human CM differentiation protocol with which high-purity ventricular-type CMs can be obtained and maintained without any CM purification process. We induced and collected a mesodermal cell population (platelet-derived growth factor receptor-α (PDGFRα)-positive cells) that can respond to CM differentiation cues, and then stimulated CM differentiation by means of Wnt inhibition. This method reproducibly generated CMs with purities above 95% in several human pluripotent stem cell lines. Furthermore, these CM populations were maintained in culture at such high purity without any further CM purification step for over 200

SUBMITTER: Fukushima H 

PROVIDER: S-EPMC7605685 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature