Intricate Genetic Programs Controlling Dormancy in Mycobacterium tuberculosis.

ABSTRACT: Mycobacterium tuberculosis (MTB) displays the remarkable ability to transition in and out of dormancy, a hallmark of the pathogen's capacity to evade the immune system and exploit susceptible individuals. Uncovering the gene regulatory programs that underlie the phenotypic shifts in MTB during disease latency and reactivation has posed a challenge. We develop an experimental system to precisely control dissolved oxygen levels in MTB cultures in order to capture the transcriptional events that unfold as MTB transitions into and out of hypoxia-induced dormancy. Using a comprehensive genome-wide transcription factor binding map and insights from network topology analysis, we identify regulatory circuits that deterministically drive sequential transitions across six transcriptionally and funct

SUBMITTER: Peterson EJR 

PROVIDER: S-EPMC7605849 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature