Downregulation of m⁶ A reader YTHDC2 promotes tumor progression and predicts poor prognosis in non-small cell lung cancer.

ABSTRACT:

Background

m⁶ A modification affects the pathological progress of many diseases by affecting RNA stability and translocation. YTHDC2, a m⁶ A reader, is associated with multiple cancers; however, little is known of its role in non-small cell lung cancer (NSCLC).

Methods

The GEPIA, Oncomine and GEO databases were analyzed to assess expression of YTHDC2 in NSCLC patients. Quantitative polymerase chain reaction, western blot and immunohistochemistry were used to detect YTHDC2 expression in different NSCLC cell lines (H1299, H460, H292 and A549) and patients. The effects of YTHDC2 on NSCLC cell lines (A549 and H1299) proliferation and migration were employed using CCK8 and transwell assays. The potential target RNAs of YTHDC2 were obtained from the POSTAR da

SUBMITTER: Sun S 

PROVIDER: S-EPMC7606000 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature