Assessment of nanoindentation in stiffness measurement of soft biomaterials: kidney, liver, spleen and uterus.

ABSTRACT: Nanoindentation technology with high spatial resolution and force sensitivity is widely used to measure the mechanical properties of hard biomaterials and tissues. However, its reliability to analyze soft biomaterials and organs has not been tested. Here, we evaluated the utility of nanoindentation to measure the passive mechanical properties of soft biological specimen. Kidney, liver, spleen and uterus samples were harvested from C57BL/6 N mice. We assessed test-retest repeatability in biological specimen and hydrogel controls using Bland-Altman diagrams, intraclass correlation coefficients (ICCs) and the within-subject coefficients of variation (COVs). The results were calculated using Hertzian, JKR and Oliver & Pharr models. Similar to hydrogels, Bland-Altman plots of all biological spe

SUBMITTER: Wu G 

PROVIDER: S-EPMC7606463 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature