Integrated Dual-Mode Chromatography to Enrich Extracellular Vesicles from Plasma.

ABSTRACT: Purifying extracellular vesicles (EVs) from complex biological fluids is a critical step in analyzing EVs molecularly. Plasma lipoprotein particles (LPPs) are a significant confounding factor as they outnumber EVs >10⁴ -fold. Given their overlap in size, LPPs cannot be completely removed using standard size-exclusion chromatography. Density-based separation of LPPs can be applied but is impractical for routine use in clinical research and practice. Here a new separation approach, known as dual-mode chromatography (DMC), capable of enriching plasma EVs, and depleting LPPs is reported. DMC conveniently integrates two orthogonal separation steps in a single column device: i) size exclusion to remove high-density lipoproteins (HDLs) that are smaller than EVs; and ii) cation exchange

SUBMITTER: Van Deun J 

PROVIDER: S-EPMC7606548 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature