The genetic Ca²⁺ sensor GCaMP3 reveals multiple Ca²⁺ stores differentially coupled to Ca²⁺ entry in the human malaria parasite Plasmodium falciparum.

ABSTRACT: Cytosolic Ca²⁺ regulates multiple steps in the host-cell invasion, growth, proliferation, and egress of blood-stage Plasmodium falciparum, yet our understanding of Ca²⁺ signaling in this endemic malaria parasite is incomplete. By using a newly generated transgenic line of P. falciparum (PfGCaMP3) that expresses constitutively the genetically encoded Ca²⁺ indicator GCaMP3, we have investigated the dynamics of Ca²⁺ release and influx elicited by inhibitors of the sarcoplasmic/endoplasmic reticulum Ca²⁺-ATPase pumps, cyclopiazonic acid (CPA), and thapsigargin (Thg). Here we show that in isolated trophozoite phase parasites: (i) both CPA and Thg release Ca²⁺ from intracellular stores in P. falciparum parasit

SUBMITTER: Borges-Pereira L 

PROVIDER: S-EPMC7606694 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature