DNA sequence analysis and Jk blood group genotype-phenotype assessment.

ABSTRACT:

Background

The Kidd (JK) blood group is critical for clinical blood transfusion. Various methods for Jk typing have been commonly used, including urea hemolysis, serological test, and genotyping. However, the application of molecular methods has so far been restricted to selected samples and not been applied to the population-scale analysis.

Methods

One hundred eighty-three blood samples, containing 174 samples collected from voluntary blood donors of Chinese Han individuals, together with 3 Jk (aw+b-) and 6 Jk (a-b-) samples, were investigated by standard serology urea hemolysis test and Sanger-sequencing. Complete coverage of exons 4-11 and intron-exon borders have been sequenced.

Results

We report the frequencies of three SNPs in exon 4, 7, and intron 9. Besides, s

SUBMITTER: Liang S 

PROVIDER: S-EPMC7607079 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature