Simultaneous two-photon activation and imaging of neural activity based on spectral-temporal modulation of supercontinuum light.

ABSTRACT:

Significance

Recent advances in nonlinear optics in neuroscience have focused on using two ultrafast lasers for activity imaging and optogenetic stimulation. Broadband femtosecond light sources can obviate the need for multiple lasers by spectral separation for chromatically targeted excitation.

Aim

We present a photonic crystal fiber (PCF)-based supercontinuum source for spectrally resolved two-photon (2P) imaging and excitation of GCaMP6s and C1V1-mCherry, respectively.

Approach

A PCF is pumped using a 20-MHz repetition rate femtosecond laser to generate a supercontinuum of light, which is spectrally separated, compressed, and recombined to image GCaMP6s (930 nm excitation) and stimulate the optogenetic protein, C1V1-mCherry (1060 nm excitation). Galvanometric spira

SUBMITTER: Liu YZ 

PROVIDER: S-EPMC7607614 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature