Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature.

ABSTRACT:

Background

Abiotic stress, including heat, is one of the major factors that affect alfalfa growth and forage yield. The small RNA, microRNA156 (miR156), regulates multiple traits in alfalfa during abiotic stress. The aim of this study was to explore the role of miR156 in regulating heat response in alfalfa at the protein level.

Results

In this study, we compared an empty vector control and miR156 overexpressing (miR156OE) alfalfa plants after exposing them to heat stress (40 °C) for 24 h. We measured physiological parameters of control and miR156OE plants under heat stress, and collected leaf samples for protein analysis. A higher proline and antioxidant contents were detected in miR156OE plants than in controls under heat stress. Protein samples were analyzed by label-free

SUBMITTER: Arshad M 

PROVIDER: S-EPMC7607685 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature