Enhanced production of taxadiene in Saccharomyces cerevisiae.

ABSTRACT:

Background

Cost-effective production of the highly effective anti-cancer drug, paclitaxel (Taxol^®), remains limited despite growing global demands. Low yields of the critical taxadiene precursor remains a key bottleneck in microbial production. In this study, the key challenge of poor taxadiene synthase (TASY) solubility in S. cerevisiae was revealed, and the strains were strategically engineered to relieve this bottleneck.

Results

Multi-copy chromosomal integration of TASY harbouring a selection of fusion solubility tags improved taxadiene titres 22-fold, up to 57 ± 3 mg/L at 30 °C at microscale, compared to expressing a single episomal copy of TASY. The scalability of the process was highlighted through achieving similar titres during scale up to 25 mL and 250 m

SUBMITTER: Nowrouzi B 

PROVIDER: S-EPMC7607689 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature