Subclinical effects of remote ischaemic conditioning in human kidney transplants revealed by quantitative proteomics.

ABSTRACT:

Background

Remote ischaemic conditioning (RIC) is currently being explored as a non-invasive method to attenuate ischaemia/reperfusion injuries in organs. A randomised clinical study (CONTEXT) evaluated the effects of RIC compared to non-RIC controls in human kidney transplants.

Methods

RIC was induced prior to kidney reperfusion by episodes of obstruction to arterial flow in the leg opposite the transplant using a tourniquet (4 × 5 min). Although RIC did not lead to clinical improvement of transplant outcomes, we explored whether RIC induced molecular changes through precision analysis of CONTEXT recipient plasma and kidney tissue samples by high-resolution tandem mass spectrometry (MS/MS).

Results

We observed an accumulation of muscle derived proteins and altered am

SUBMITTER: Thorne AM 

PROVIDER: S-EPMC7607690 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature