Molecular characterization of canine astrovirus, vesivirus and circovirus, isolated from diarrheic dogs in Turkey.

ABSTRACT:

Background

Canine astrovirus (CAstV) has been considered the primary cause of gastroenteritis in young animals worldwide, while canine vesivirus (CVeV) and canine circovirus (CCiV) are occasionally reported.

Aims

This study aimed to investigate the existence and molecular characteristics for these three viruses in Turkey. Methods: Faecal samples from 150 shelter dogs with gastrointestinal problems (127 adults and 23 puppies) were collected and examined by reverse transcription-polymerase chain reaction (RT-PCR) analysis based on the partial sequence of RdRp gene (ORF1b) for CAstV, ORF2 gene of CVeV and capsid protein (Cap) and replication associated protein (Rep) gene of CCiV. Randomly selected positive samples were submitted to sequencing and mol

SUBMITTER: Turan T 

PROVIDER: S-EPMC7608039 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature