Mechanisms Regulating Muscle Protein Synthesis in CKD.

ABSTRACT:

Background

CKD induces loss of muscle proteins partly by suppressing muscle protein synthesis. Muscles of mice with CKD have increased expression of nucleolar protein 66 (NO66), as do muscle biopsy specimens from patients with CKD or those undergoing hemodialysis. Inflammation stimulates NO66 expression and changes in NF-κB mediate the response.

Methods

Subtotal nephrectomy created a mouse model of CKD with BUN >80 mg/dl. Crossing NO66^flox/flox with MCK-Cre mice bred muscle-specific NO66 (MCK-NO66) knockout mice. Experiments assessed the effect of removing NO66.

Results

Muscle-specific NO66 knockout in mice blocks CKD-induced loss of muscle mass and improves protein synthesis. NO66 suppression of ribosomal biogenesis via demethylase activity i

SUBMITTER: Zhang L 

PROVIDER: S-EPMC7608956 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature