Coordinated Circulating T Follicular Helper and Activated B Cell Responses Underlie the Onset of Antibody-Mediated Rejection in Kidney Transplantation.

ABSTRACT:

Background

Although antibody-mediated rejection (ABMR) has been long recognized as a leading cause of allograft failure after kidney transplantation, the cellular and molecular processes underlying the induction of deleterious donor-specific antibody (DSA) responses remain poorly understood.

Methods

Using high-dimensional flow cytometry, in vitro assays, and RNA sequencing, we concomitantly investigated the role of T follicular helper (T_FH) cells and B cells during ABMR in 105 kidney transplant recipients.

Results

There were 54 patients without DSAs; of those with DSAs, ABMR emerged in 20 patients, but not in 31 patients. We identified proliferating populations of circulating T_FH cells and activated B cells emerging in blood of patients un

SUBMITTER: Louis K 

PROVIDER: S-EPMC7608995 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature