Identification of Theaflavin-3,3'-Digallate as a Novel Zika Virus Protease Inhibitor.

ABSTRACT: Mounting evidence indicates that Zika virus (ZIKV) is closely related to neurological disorders such as microcephaly and Guillain-Barré syndrome. There are currently no effective vaccines and FDA-approved inhibitors against ZIKV infection. The flaviviral heterodimeric serine protease NS2B-NS3 plays an essential role in ZIKV maturation and replication, thus becoming a promising target in anti-ZIKV therapy. Herein, we developed a fluorescence-based screening assay to search for inhibitors targeting the ZIKV NS2B-NS3 protease (ZIKVpro), and identified theaflavin-3,3'-digallate (ZP10), a natural active compound derived from black tea, as a potent ZIKV protease inhibitor in vitro (IC_{50 =} 2.3 μM). ZP10 exhibited dose-dependent inhibitory effect on ZIKV replication (EC_{50 =<}

SUBMITTER: Cui X 

PROVIDER: S-EPMC7609463 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature