Efficient population modification gene-drive rescue system in the malaria mosquito Anopheles stephensi.

ABSTRACT: Cas9/gRNA-mediated gene-drive systems have advanced development of genetic technologies for controlling vector-borne pathogen transmission. These technologies include population suppression approaches, genetic analogs of insecticidal techniques that reduce the number of insect vectors, and population modification (replacement/alteration) approaches, which interfere with competence to transmit pathogens. Here, we develop a recoded gene-drive rescue system for population modification of the malaria vector, Anopheles stephensi, that relieves the load in females caused by integration of the drive into the kynurenine hydroxylase gene by rescuing its function. Non-functional resistant alleles are eliminated via a dominantly-acting maternal effect combined with slower-acting standard negative sel

SUBMITTER: Adolfi A 

PROVIDER: S-EPMC7609566 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature