Identification of mesenchymal stromal cell survival responses to antimicrobial silver ion concentrations released from orthopaedic implants.

ABSTRACT: Antimicrobial silver (Ag⁺) coatings on orthopaedic implants may reduce infection rates, but should not be to the detriment of regenerative cell populations, primarily mesenchymal stem/stromal cells (MSCs). We determined intramedullary silver release profiles in vivo, which were used to test relevant Ag⁺ concentrations on MSC function in vitro. We measured a rapid elution of Ag⁺ from intramedullary pins in a rat femoral implantation model, delivering a maximum potential concentration of 7.8 µM, which was below toxic levels determined for MSCs in vitro (EC₅₀, 33 µM). Additionally, we present in vitro data of the reduced colonisation of implants by Staphylococcus aureus. MSCs exposed to Ag⁺ prior to/during osteogenic differentiation were

SUBMITTER: Souter P 

PROVIDER: S-EPMC7609692 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature