Addressing Differentiation in Live Human Keratinocytes by Assessment of Membrane Packing Order.

ABSTRACT: Differentiation of keratinocytes is critical for epidermal stratification and formation of a protective stratum corneum. It involves a series of complex processes leading through gradual changes in characteristics and functions of keratinocytes up to their programmed cell death via cornification. The stratum corneum is a relatively impermeable barrier, comprised of dead cell remnants (corneocytes) embedded in lipid matrix. Corneocyte membranes are comprised of specialized lipids linked to late differentiation proteins, contributing to the formation of a stiff and mechanically strengthened layer. To date, the assessment of the progression of keratinocyte differentiation is only possible through determination of specific differentiation markers, e.g., by using proteomics-based

SUBMITTER: Gutowska-Owsiak D 

PROVIDER: S-EPMC7609878 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature