Ectopic humanized mesenchymal niche in mice enables robust engraftment of myelodysplastic stem cells.

ABSTRACT: Myelodysplastic syndrome (MDS) are clonal stem cell diseases characterized mainly by ineffective hematopoiesis. Here, we present an approach that enables robust long-term engraftment of primary MDS stem cells (MDS-SCs) in mice by implantation of human mesenchymal cell-seeded scaffolds. Critically for modelling MDS, where patient sample material is limiting, mononuclear bone marrow cells containing as few as 10⁴ CD34⁺ cells can be engrafted and expanded by this approach with the maintenance of the genetic make-up seen in the patients. Non-invasive high-resolution ultrasound imaging shows that these scaffolds are fully perfused. Our data shows that human microenvironment but not mouse is essential to MDS-SCs homing and engraftment. Notably, the alternative niche provide

SUBMITTER: Mian SA 

PROVIDER: S-EPMC7610449 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature