Genomic encoding of transcriptional burst kinetics.

ABSTRACT: Mammalian gene expression is inherently stochastic^1,2, and results in discrete bursts of RNA molecules that are synthesized from each allele^3-7. Although transcription is known to be regulated by promoters and enhancers, it is unclear how cis-regulatory sequences encode transcriptional burst kinetics. Characterization of transcriptional bursting, including the burst size and frequency, has mainly relied on live-cell^4,6,8 or single-molecule RNA fluorescence in situ hybridization^3,5,8,9 recordings of selected loci. Here we determine transcriptome-wide burst frequencies and sizes for endogenous mouse and human genes using allele-sensitive single-cell RNA sequencing. We show that core promoter elements affect burst size and uncover synergistic effect

SUBMITTER: Larsson AJM 

PROVIDER: S-EPMC7610481 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature