Site-specific covalent labeling of His-tag fused proteins with N-acyl-N-alkyl sulfonamide reagent.

ABSTRACT: The ability to incorporate a desired functionality into proteins of interest in a site-specific manner can provide powerful tools for investigating biological systems and creating therapeutic conjugates. However, there are not any universal methods that can be applied to all proteins, and it is thus important to explore the chemical strategy for protein modification. In this paper, we developed a new reactive peptide tag/probe pair system for site-specific covalent protein labeling. This method relies on the recognition-driven reaction of a peptide tag and a molecular probe, which comprises the lysine-containing short histidine tag (KH6 or H6K) and a binuclear nickel (II)- nitrilotriacetic acid (Ni²⁺-NTA) complex probe containing a lysine-reactive N-acyl-N-alkyl sulfonamide (NA

SUBMITTER: Thimaradka V 

PROVIDER: S-EPMC7610637 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature