Proteomic characterization of the normal human medial meniscus body using data-independent acquisition mass spectrometry.

ABSTRACT: Recent research suggests an important role of the meniscus in the development of knee osteoarthritis. We, therefore, aimed to analyze the proteome of the normal human meniscus body, and specifically to gain new knowledge on global protein expression in the different radial zones. Medial menisci were retrieved from the right knees of 10 human cadaveric donors, from which we cut a 2 mm radial slice from the mid-portion of the meniscal body. This slice was further divided into three zones: inner, middle, and peripheral. Proteins were extracted and prepared for mass spectrometric analysis using data-independent acquisition. We performed subsequent data searches using Spectronaut Pulsar and used fixed-effect linear regression models for statistical analysis. We identified 638 proteins and after

SUBMITTER: Folkesson E 

PROVIDER: S-EPMC7610686 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature