Three-dimensional microstructure of human meniscus posterior horn in health and osteoarthritis.

ABSTRACT:

Objective

To develop and perform ex vivo 3D imaging of meniscus posterior horn microstructure using micro-computed tomography (μCT), and to compare specimens from healthy references against end-stage osteoarthritis (OA) using conventional section-based histology and qualitative μCT.

Design

We retrieved human medial and lateral menisci from 10 deceased donors without knee OA (healthy references) and medial and lateral menisci from 10 patients having total knee replacement for medial compartment OA. Meniscal posterior horns were dissected and fixed in formalin. One subsection underwent hexamethyldisilazane (HMDS) treatment and μCT imaging. Pauli's histopathological scoring was performed for 3 other subsections. The differences in histopathological scores were estimated using m

SUBMITTER: Kestila I 

PROVIDER: S-EPMC7610688 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature