Community-Wide Experimental Evaluation of the PROSS Stability-Design Method.

ABSTRACT: Recent years have seen a dramatic improvement in protein-design methodology. Nevertheless, most methods demand expert intervention, limiting their widespread adoption. By contrast, the PROSS algorithm for improving protein stability and heterologous expression levels has been successfully applied to a range of challenging enzymes and binding proteins. Here, we benchmark the application of PROSS as a stand-alone tool for protein scientists with no or limited experience in modeling. Twelve laboratories from the Protein Production and Purification Partnership in Europe (P4EU) challenged the PROSS algorithm with 14 unrelated protein targets without support from the PROSS developers. For each target, up to six designs were evaluated for expression levels and in some cases, for thermal stability

SUBMITTER: Peleg Y 

PROVIDER: S-EPMC7610701 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature