RNA origami design tools enable cotranscriptional folding of kilobase-sized nanoscaffolds.

ABSTRACT: RNA origami is a framework for the modular design of nanoscaffolds that can be folded from a single strand of RNA and used to organize molecular components with nanoscale precision. The design of genetically expressible RNA origami, which must fold cotranscriptionally, requires modelling and design tools that simultaneously consider thermodynamics, the folding pathway, sequence constraints and pseudoknot optimization. Here, we describe RNA Origami Automated Design software (ROAD), which builds origami models from a library of structural modules, identifies potential folding barriers and designs optimized sequences. Using ROAD, we extend the scale and functional diversity of RNA scaffolds, creating 32 designs of up to 2,360 nucleotides, five that scaffold two proteins, and seven that scaffo

SUBMITTER: Geary C 

PROVIDER: S-EPMC7610888 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature