Determining bacterial and host contributions to the human salivary metabolome.

ABSTRACT:

Background

Salivary metabolomics is rapidly advancing.

Aim and methods

To determine the extent to which salivary metabolites reflects host or microbial metabolic activity whole-mouth saliva (WMS), parotid saliva (PS) and plasma collected contemporaneously from healthy volunteers were analysed by ¹H-NMR spectroscopy. Spectra underwent principal component analysis and k-means cluster analysis and metabolite quantification. WMS samples were cultured on both sucrose and peptide-enriched media. Correlation between metabolite concentration and bacterial load was assessed.

Results

WMS contained abundant short-chain fatty acids (SCFAs), which were minimal in PS and plasma. WMS spectral exhibited greater inter-individual variation than those of PS or plasma (6.7 and

SUBMITTER: Gardner A 

PROVIDER: S-EPMC7610937 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature