Structure of human telomerase holoenzyme with bound telomeric DNA.

ABSTRACT: Telomerase adds telomeric repeats at chromosome ends to compensate for the telomere loss that is caused by incomplete genome end replication¹. In humans, telomerase is upregulated during embryogenesis and in cancers, and mutations that compromise the function of telomerase result in disease². A previous structure of human telomerase at a resolution of 8 Å revealed a vertebrate-specific composition and architecture³, comprising a catalytic core that is flexibly tethered to an H and ACA (hereafter, H/ACA) box ribonucleoprotein (RNP) lobe by telomerase RNA. High-resolution structural information is necessary to develop treatments that can effectively modulate telomerase activity as a therapeutic approach against cancers and disease. Here we used cryo-electron

SUBMITTER: Ghanim GE 

PROVIDER: S-EPMC7610991 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature