INDIANA: An in-cell diffusion method to characterize the size, abundance and permeability of cells.

ABSTRACT: NMR and MRI diffusion experiments contain information describing the shape, size, abundance, and membrane permeability of cells although extracting this information can be challenging. Here we present the INDIANA (IN-cell DIffusion ANAlysis) method to simultaneously and non-invasively measure cell abundance, effective radius, permeability and intrinsic relaxation rates and diffusion coefficients within the inter- and intra-cellular populations. The method couples an experimental dataset comprising stimulated-echo diffusion measurements, varying both the gradient strength and the diffusion delay, together with software to fit a model based on the Kärger equations to robustly extract the relevant parameters. A detailed error analysis is presented by comparing the results from fitting simulat

SUBMITTER: Karunanithy G 

PROVIDER: S-EPMC7611012 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature