Responsive fluorescent nucleotides serve as efficient substrates to probe terminal uridylyl transferase.
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ABSTRACT: We repurposed a terminal uridylyl transferase enzyme to site-specifically label RNA with microenvironment sensing fluorescent nucleotide mimics, which in turn provided direct read-outs to estimate the binding affinities of the enzyme to RNA and nucleotide substrates. This enzyme-probe system provides insights into the catalytic cycle, and can facilitate the development of discovery platforms to identify robust enzyme inhibitors.
SUBMITTER: George JT
PROVIDER: S-EPMC7611084 | biostudies-literature |
REPOSITORIES: biostudies-literature
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