Native detection of protein <i>O</i>-GlcNAcylation by gel electrophoresis.

ABSTRACT: O-GlcNAcylation is an abundant and dynamic protein posttranslational modification (PTM), with crucial roles in metazoans. Studies of this modification are hampered by the lack of convenient methods for detecting native O-GlcNAcylation. Here, we describe a novel gel-based approach, Separation of O-GlcNAcylated Proteins by Polyacrylamide Gel Electrophoresis (SOPAGE), which enables detection of O-GlcNAc levels and dynamics.

SUBMITTER: Fu C 

PROVIDER: S-EPMC7611126 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature