Optimal His-Tag Design for Efficient [<sup>99m</sup>Tc(CO)<sub>3</sub>]<sup>+</sup> and [<sup>188</sup>Re(CO)<sub>3</sub>]<sup>+</sup> Labeling of Proteins for Molecular Imaging and Radionuclide Therapy by Analysis of Peptide Arrays.
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ABSTRACT: Hexahistidine tags (His-tags), incorporated into recombinant proteins to facilitate purification using metal-affinity chromatography, are useful binding sites for radiolabeling with [99mTc(CO)3]+ and [188Re(CO)3]+ for molecular imaging and radionuclide therapy. Labeling efficiencies vary unpredictably, and the method is therefore not universally useful. To overcome this, we have made quantitative comparisons of radiolabeling of a bespoke Celluspots array library of 382 His-tag-containing peptide sequences with [99mTc(CO)3]+ and [188Re(CO)3]+ to identify key features that enhance labeling. A selected sequence with 10-fold enhanced labeling efficiency compared t
SUBMITTER: Williams JD
PROVIDER: S-EPMC7611356 | biostudies-literature | 2021 Jul
REPOSITORIES: biostudies-literature
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