Protein Topology Prediction Algorithms Systematically Investigated in the Yeast Saccharomyces cerevisiae.

ABSTRACT: Membrane proteins perform a variety of functions, all crucially dependent on their orientation in the membrane. However, neither the exact number of transmembrane domains (TMDs) nor the topology of most proteins have been experimentally determined. Due to this, most scientists rely primarily on prediction algorithms to determine topology and TMD assignments. Since these can give contradictory results, single-algorithm-based predictions are unreliable. To map the extent of potential misanalysis, the predictions of nine algorithms on the yeast proteome are compared and it is found that they have little agreement when predicting TMD number and termini orientation. To view all predictions in parallel, a webpage called TopologYeast: http://www.weizmann.ac.il/molgen/TopologYeast was created. Eac

SUBMITTER: Weill U 

PROVIDER: S-EPMC7611422 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature