Transcriptome-wide dynamics of extensive m⁶A mRNA methylation during Plasmodium falciparum blood-stage development.

ABSTRACT: Malaria pathogenesis results from the asexual replication of Plasmodium falciparum within human red blood cells, which relies on a precisely timed cascade of gene expression over a 48-h life cycle. Although substantial post-transcriptional regulation of this hardwired program has been observed, it remains unclear how these processes are mediated on a transcriptome-wide level. To this end, we identified mRNA modifications in the P. falciparum transcriptome and performed a comprehensive characterization of N⁶-methyladenosine (m⁶A) over the course of blood-stage development. Using mass spectrometry and m⁶A RNA sequencing, we demonstrate that m⁶A is highly developmentally regulated, exceeding m⁶A levels known in any other eukaryote. We cha

SUBMITTER: Baumgarten S 

PROVIDER: S-EPMC7611496 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature