Niche-Mediated Integrin Signaling Supports Steady-State Hematopoiesis in the Spleen.

ABSTRACT: Outside-in integrin signaling regulates cell fate decisions in a variety of cell types, including hematopoietic stem cells (HSCs). Our earlier published studies showed that interruption of periostin (POSTN) and integrin-αv (ITGAV) interaction induces faster proliferation in HSCs with developmental stage-dependent functional effects. In this study, we examined the role of POSTN-ITGAV axis in lymphohematopoietic activity in spleen that hosts a rare population of HSCs, the functional regulation of which is not clearly known. Vav-iCre-mediated deletion of Itgav in the hematopoietic system led to higher proliferation rates, resulting in increased frequency of primitive HSCs in the adult spleen. However, in vitro CFU-C assays demonstrated a poorer differentiation potential followin

SUBMITTER: Mehatre SH 

PROVIDER: S-EPMC7611631 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature