Mechanical compartmentalization of the intestinal organoid enables crypt folding and collective cell migration.

ABSTRACT: Intestinal organoids capture essential features of the intestinal epithelium such as crypt folding, cellular compartmentalization and collective movements. Each of these processes and their coordination require patterned forces that are at present unknown. Here we map three-dimensional cellular forces in mouse intestinal organoids grown on soft hydrogels. We show that these organoids exhibit a non-monotonic stress distribution that defines mechanical and functional compartments. The stem cell compartment pushes the extracellular matrix and folds through apical constriction, whereas the transit amplifying zone pulls the extracellular matrix and elongates through basal constriction. The size of the stem cell compartment depends on the extracellular-matrix stiffness and endogenous cellular fo

SUBMITTER: Perez-Gonzalez C 

PROVIDER: S-EPMC7611697 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature